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> Electromyography (EMG) problem
StijnS
post Mar 27, 2013, 07:24 AM
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Dear all,

I'm currently trying to determine a motor threshold for a Transcranial Magnetic Stimulation (TMS) experiment in an anaesthetized rat. However, I'm having difficulties getting a decent baseline. Instead of a clear baseline with little noise when there is no activity or stimulation, I have a constant sinuswave on the monitor with too much noise to detect any small changes.

Since I suspected this had to do with the ground electrode, I have tried several locations (needle electrode in the tail, needle electrode subcutaneously in the back, surface electrode on the back), unfortunately each without succes.

The anode (reference) is placed on the foot of the rat. Here I tried a needle electrode between the second and third digit as well.

Also for the muscle itself I tried the front paw and the hind limb with surface and needle electrodes, without succes.

The sensitivity of the monitor is set at increments of 50 uV, while in literature increments of 15 - 50 uV in amplitude (peak-to-peak) should be detectable.
Since this is often done in articles, this should be accomplishable.

Does anyone here have any experience with this or any suggestions on what the problem might be?

Many thanks,
Stijn
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