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> Question about stimulator not working in whole cell patch clamp
Jun
post Jul 21, 2011, 08:45 AM
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Hi Guys,

I have a prob and it'd be great if somebody could give me an idea how to solve it or just throw an idea!!

So I've been doing voltage patch clamping, whole cell recording. I can record beautiful mIPSC/EPSC. Now I want to record evoked IPSC so I'm using a stimulator injecting current. I saw the artifact and it goes up and down to the intensity of my stimulation. I checked the connectivity of the system and it's all fine. I put the stimulator fairly close to my neuron and it should work. But I just can't see a response IPSC/EPSC. When I put only the stimulator, patch pipette and ground in the bath, I also only see the artifact, no current.

Anybody knows what's happening here possily, please?

Thank you!

Jun
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