Artificial Cerebral Spinal Fluid Recipe, Need an ACSF that have a high level of background activity in neurons Options

[coolfez]

Hi everyone,

I am in the midest of designing a new experiment using slices of rat brain. I know there are many ACSF recipes out there. My PI told me of a recipe that he came across back in the day ( 1990?) where the ACSF used on the slice did not dampen the background activity of the neurons. In fact, I think it actually made them go up.


The ACSF in my lab right now is the kind that keeps the background activity very low. If someone could let me know of an recipe for an ACSF that will keep and/raise background activity in neurons that would be great! It would be even better if you could go into the Neurochemistry explanation of why. Thanks!

[Debbie]

Hi, be careful with ACSFs, latest info is that the regular one based on glucose can make slices energy deficient.

Free full text is at Frontiersin . org, go to Science -> Neuroscience -> Neuroenergetics and see the article titled "Lactate effectively covers energy demands during neuronal network activity in neonatal hippocampal slices"

Hi everyone,

I am in the midest of designing a new experiment using slices of rat brain. I know there are many ACSF recipes out there. My PI told me of a recipe that he came across back in the day ( 1990?) where the ACSF used on the slice did not dampen the background activity of the neurons. In fact, I think it actually made them go up.


The ACSF in my lab right now is the kind that keeps the background activity very low. If someone could let me know of an recipe for an ACSF that will keep and/raise background activity in neurons that would be great! It would be even better if you could go into the Neurochemistry explanation of why. Thanks!